Sweet-tasting protein brazzein mutant having high sweetness and preparation method therefor

Abstract

The present invention provides a method for preparing Brazzein or a mutant thereof, comprising: 1) constructing an expression vector, the expression vector comprising a coding sequence of the Brazzein or the mutant thereof and a coding sequence of a leader peptide, wherein the leader peptide is derived from Rhizopus oryzae lipase; and 2) introducing the expression vector into a host cell. The present invention further provides Brazzein or a mutant thereof prepared by the method. The sweetness of the Brazzein or the mutant thereof is significantly higher than that of Brazzein prepared in the prior art, and the expressed protein has no obvious sweetness delay.

Dependencies are inferred from the claim text.