Compositions and methods for treating viral infections
AssigneeG Tech Bio LLC
InventorSerhat Gumrukcu
Life of this patent
Estimating the term…
Abstract
The disclosure provides methods and compositions utilizing recombinant nucleic acid constructs or a replication incompetent virus-like particle encoding a chemokine, cytokine, or apoptosis inducing protein (e.g. Caspase 9 (Casp9)), or other toxins in a form which can only be transcribed in the presence of a viral polymerase. These methods can be adapted to target many viral infections and reduce or eliminate viral load, and provide a fundamentally different treatment for viral infections.
Claims12
1-52. (canceled)
A recombinant nucleic acid molecule comprising:
- a negative strand nucleic acid molecule or a pgRNA nucleic acid molecule encoding a chemokine, a cytokine, an apoptosis inducing protein, diphtheria toxin A (or a fragment thereof), or any combination thereof flanked by a first and second viral transcription recognition signal;
- a first promoter upstream (5′) of the first viral transcription recognition signal and
- a second promoter adjacent and 5′ to the negative strand nucleic acid molecule or pgRNA nucleic acid molecule encoding a chemokine, a cytokine, an apoptosis inducing protein, diphtheria toxin A (or a fragment thereof), or any combination thereof.
A replication incompetent virus-like particle (VLP) comprising:
- an optional translocation motif (TLM) fused to a capsid protein from a hepatitis B viral capsid or a hepatitis D viral capsid or coronavirus fusion protein;
- a negative strand nucleic acid molecule or a pgRNA nucleic acid molecule encoding a chemokine, a cytokine, a apoptosis inducing protein, diphtheria toxin A (or a fragment thereof), or any combination thereof, flanked by a first and second viral transcription recognition signal;
- a first promoter upstream (5′) of the first viral transcription recognition signal;
- and
- a second promoter adjacent and 5′ to the negative strand nucleic acid molecule or pgRNA nucleic acid molecule encoding a chemokine, a cytokine, a apoptosis inducing protein, diphtheria toxin A (or a fragment thereof), or any combination thereof.
A replication incompetent virus-like particle (VLP) comprising:
- a negative strand nucleic acid molecule or a pgRNA nucleic acid molecule encoding a chemokine, a cytokine, a apoptosis inducing protein, diphtheria toxin A (or a fragment thereof), or any combination thereof, flanked by a first and second target viral transcription recognition signal;
- a first promoter upstream (5′) of the first viral transcription recognition signal;
- and
- a second promoter adjacent and 5′ to the negative strand nucleic acid molecule or pgRNA nucleic acid molecule encoding a chemokine, a cytokine, a apoptosis inducing protein, diphtheria or toxin A (or fragment thereof), or any combination thereof, wherein the VLP exhibits tropism for the target virally infected cells.
Dependencies are inferred from the claim text.