Unnatural sugar, and synthesis method therefor and use thereof

Abstract

Disclosed in the present invention are an unnatural sugar, and a synthesis method therefor and the use thereof. The synthesis method comprises: subjecting hydroxyl groups of an amino sugar to per-trimethylsilane protection at room temperature, selectively exposing amino groups on the sugar, and coupling and converting the amino groups at room temperature to obtain a per-trimethylsilane-protected unnatural sugar with orthogonal groups; and removing trimethylsilane protecting groups from the per-trimethylsilane-protected unnatural sugar with orthogonal groups to obtain an unnatural sugar without protecting groups. The present invention takes the advantages of existing unnatural sugars into consideration, such that not only is it ensured that the unnatural sugar can be efficiently utilized by cells, but an S side reaction of the unnatural sugar with cysteine in protein during the metabolic process thereof is also effectively avoided; in addition, efficient metabolic marking is achieved. In a cell test, the usage concentration of a 1,6-bis-acylated unnatural sugar is one order of magnitude lower than that of the unnatural sugar without protecting groups.

Dependencies are inferred from the claim text.