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US 2026/0208143 A1Published application
Electrochemical polynucleotide synthesis
AssigneeTwist Bioscience Corporation
InventorsJeremy LACKEY, Maryam HABIBIAN, YanYou LIN
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Abstract
Provided herein are compositions, devices, systems and methods for generation and use of biomolecule-based information for storage. Further provided are devices comprising addressable electrodes controlling polynucleotide synthesis (deprotection, extension, or cleavage, etc.) Further provided are compositions for low voltage deprotection of polynucleotides.
Claims21
1-36. (canceled)
A method for polynucleotide synthesis comprising:
- contacting a nucleoside attached to a solid support with a protected nucleoside, wherein the protected nucleoside is configured to form a covalent bond with the nucleoside to generate a protected polynucleotide comprising an acid cleavable protecting group;
- contacting the protected polynucleotide with a composition comprising a first redox compound, and a second redox compound, wherein a redox potential between the first redox compound and the second redox compound is less than 2 volts;
- and
- applying, in a series of pulses, a voltage to a solvent in fluid communication with the protected polynucleotide, wherein the voltage results in deprotection of a terminal nucleoside of the protected polynucleotide.
1 more element
Dependencies are inferred from the claim text.