Method for producing gel-like wound healing agent, gel-like wound healing agent, and autologous platelet-rich plasma gel preparation kit for wound healing

A method for producing a wound healing gel sheet, comprising mixing:

• (A) an effective amount of platelet-rich plasma,
• (B) an ascorbic acid solution at a concentration of 150 to 300 mg/mL,
• (C) a calcium chloride solution at a concentration of 1.0 to 3.0 wt. %, and
• (D) thrombin,
• wherein
• a mixing ratio between (A) platelet-rich plasma and (B) ascorbic acid solution is

A wound healing gel obtained by mixing:

• (A) an effective amount of platelet-rich plasma,
• (B) an ascorbic acid solution at a concentration of 150 to 300 mg/mL,
• (C) a calcium chloride solution at a concentration of 1.0 to 3.0 wt. %, and
• (D) thrombin,
• wherein
• a mixing ratio between (A) platelet-rich plasma and (B) ascorbic acid solution is 1.0 to 3.0 mL of (B) ascorbic acid solution per 8.0 mL of (A) platelet-rich plasma, and
• a mixing ratio between (A) platelet-rich plasma and (C) calcium chloride
• solution is 1.0 to 3.0 mL of (C) calcium chloride solution per 8.0 mL of (A) platelet-rich plasma.

An autologous platelet-rich plasma gel preparation kit for wound healing, consisting of:

• (I) blood collection needle,
• (II) blood collection tube, and
• (III) gelation reagent set,
• wherein (III) gelation reagent set contains ascorbic acid solution at a concentration of 150 to 300 mg/mL, calcium chloride solution at a concentration of 1.0 to 3.0 wt. %, and thrombin in separate forms.