Oxygen enema composition, enema device, and method for producing oxygen enema composition

A method of treatment including a step of supplying an oxygen enema composition containing an oxygen dissolved solution to an intestine from an anus of a mammal, wherein the oxygen dissolved solution has an amount of dissolved oxygen of 50 ppm or more, wherein the amount of dissolved oxygen is determined by a difference (P1−P2) between a numerical value P1 of an amount of dissolved oxygen (ppm) measured by a dissolved oxygen measurement,

• the method comprising the steps of:
• (1) adding a deoxidizing amino compound to an oxygen dissolved solution to obtain a mixture, and heating the mixture at a temperature of 80° C. or more;
• (2) measuring a concentration of the deoxidizing amino compound contained in the heated mixture after the step (1);
• and
• (3) calculating an amount of dissolved oxygen contained in the oxygen dissolved solution before passing through the step (1) from a concentration of the deoxidizing amino compound measured in the step (2), and
• a numerical value P2 of an amount of dissolved oxygen (ppm) of an oxygen dissolved solution measured by a dissolved oxygen measurement method of any one of a diaphragm electrode process, a fluorescence method, and a Winkler method.