Method for isolating plant stem cells from plant leaves and the associated cell lines obtained utilising the method
AssigneeGreen Bioactives Limited
InventorsYuan LI, Samuel CASASOLA-ZAMORA, Gary J. LOAKE, David MCELROY
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Abstract
Disclosed herein is a method of isolating a stem cell line derived from a tissue from a primary meristem in a leaf of a plant, including the steps of sterilizing at least a part of the leaf containing the central or other vein; cutting through the central or other vein of the leaf to expose primary meristematic cells from the central or other vein inner vascular tissue; and culturing cells from said exposed cells, which circumvents the difficulties of surface sterilization when other types of explants are used. Also disclosed herein are stem cell lines obtained using the method.
Claims24
A method of obtaining at least one plant stem cell, the method comprising:
- sterilizing at least a part of a leaf containing a central or other vein;
- dissecting the central or other vein of the leaf to expose primary meristematic cells from the central or other vein vascular tissue;
- and
- obtaining at least one stem cell from the central or other vein inner vascular tissue.
A stem cell line derived from a primary meristem in a leaf of a plant, wherein the stem cell line is formed of undifferentiated cells, preferably substantially genetically homogenous cells, wherein
- cells of the stem cell line have a morphology with multiple amyloplasts, wherein the stem cell line is fast-growing, and
- optionally, wherein the stem cell line has a high phytochemical accumulation in response to elicitation.
A method for producing a plant natural product or extract, the method comprising:
- isolating at least one stem cell derived from a tissue from a primary meristem in a leaf of a plant, wherein preferably the at least one stem cell is derived from primary meristematic cells from the central or other vein vascular tissue;
- and
- culturing the at least one stem cell to establish at least one stem cell line, preferably wherein the stem cell line is substantially genetically homogenous;
- optionally selecting a high-performance cell line;
- and/or
- optionally, eliciting the production or secretion of a plant natural product or extract;
- and
- collecting the plant natural product or extract.
Dependencies are inferred from the claim text.