Methods for producing soybean with altered composition

A method for producing a soybean plant having high seed oil, the method comprising:

• a. genotyping a soybean population comprising a plurality of soybean plants or soybean germplasm for the presence of at least one marker genetically linked to a locus comprising or corresponding to an MFT gene having at least 95% identity to SEQ ID NO:
• 7, the at least one marker detecting a modification in the MFT gene;
• b. selecting from the soybean population one or more soybean plants or soybean germplasm comprising the at least one marker;
• and
• c. crossing the selected soybean plant or soybean germplasm with a second soybean plant or soybean germplasm to produce a progeny population, wherein at least one soybean plant or soybean germplasm of the progeny population comprises the at least one marker and has high seed oil as compared to a seed of a control plant.

A method of introgressing a high soybean seed oil MFT allele into a soybean plant, the method comprising:

• a. crossing a first soybean plant with a second soybean plant to produce a progeny population, the first soybean plant comprising a high soybean seed oil MFT allele comprising a modification of an MFT gene comprising a polynucleotide sequence having at least 95% sequence identity with SEQ ID NO:
• 7, the modification decreasing the expression or activity of an MFT polypeptide encoded by the MFT gene;
• b. genotyping the progeny population for the presence of at least one marker genetically linked to the high soybean seed oil MFT allele;
• and
• c. selecting progeny that comprise the at least one marker to obtain soybean plants comprising the high soybean seed oil MFT allele.