Detection of Target Molecules in Dried Biologic Matrices
AssigneeSomaLogic Operating Co., Inc.
InventorsWeston J. Smith, Daniel W. Drolet
Life of this patent
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Abstract
Methods for detection of target molecules in dried biologic matrices in proteomic based assays are provided. Such methods have a wide utility in proteomic applications for research and development, diagnostics and therapeutics by providing methods for the preparation of dried biologic matrices for detection of target molecules in a proteomic assay and to methods of detecting one or more target molecules from dried biologic matrices in a proteomic assay.
Claims31
A method of preparing a biological sample for a multiplex assay comprising:
- depositing the biological sample comprising a plurality of target molecules onto a collection device;
- drying the biological sample on the collection device for a period of time to stabilize the dried sample prior to any temperature fluctuation;
- wherein the biological sample is dried for at least 4 hours at about room temperature or below room temperature so that target molecules in the dried biological sample are detectable in the multiplex assay.
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A method of detecting a plurality of target molecules comprising:
- extracting target molecules in a dried biological sample from a collection device;
- diluting the extracted target molecules into a first dilution and a second dilution;
- contacting the first dilution with a first capture reagent to form a first capture reagent affinity complex with its target molecule if the target molecule is present in the first dilution;
- contacting the second dilution with a second capture reagent to form a second capture reagent affinity complex with its target molecule if the target molecule is present in the second dilution;
- incubating the first and second dilution samples separately to allow capture reagent affinity complex formation;
- wherein each of the first capture reagent affinity complex and the second capture reagent affinity complex are immobilized on separate first solid supports;
- releasing and capturing the first capture reagent affinity complex on a second solid support;
- after releasing the first capture reagent affinity complex, releasing and capturing the second capture reagent affinity complex on the second solid support;
- and
- detecting for the presence of or determining the level of the first capture reagent, second capture reagent of the first or second capture reagent affinity complexes, or the presence or amount of the first or second capture reagent affinity complexes.
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18-34. (canceled)
38-63. (canceled)
65-81. (canceled)
A method for preparing a liquid sample comprising:
- drying the sample at a constant temperature of from −20° C. to room temperature for at least four (4) hours to create a dried sample;
- and
- reconstituting the dried sample with a formulation comprising a buffering agent, one or more salts, a chelating agent, a protease inhibitor, a non-ionic surfactant, and an oligonucleotide.
84-110. (canceled)
112-124. (canceled)
A composition comprising:
- dried sample and a formulation comprising a buffering agent, one or more salts, a chelating agent, a protease inhibitor, a non-ionic surfactant, and an oligonucleotide;
- wherein, the dried sample was derived from a liquid sample dried at a constant temperature of from about −20° C. to room temperature for at least four (4) hours.
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Dependencies are inferred from the claim text.