Extracellular vesicles, isolation and use as biomarkers
AssigneeThe University of Newcastle
InventorsMurray John CAIRNS, Michelle Mia BARNETT
Life of this patent
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Abstract
The present disclosure relates to neuron derived extracellular vesicles and methods for their isolation as well as their use as biomarkers, including for neurodegenerative and/or neuropsychiatric diseases. The present disclosure also relates to identification of small non-coding RNA and their use for diagnosis and/or prognosis and/or therapy stratification.
Claims78
A method of diagnosing a disease state of a subject, said method comprising the steps of:
- a) measuring the level of one or more small non-coding RNA in a sample from the subject, wherein the one or more small non-coding RNA comprises a nucleotide sequence shown in any one of SEQ ID NOs:
- 1 to 29, and wherein, the sample comprises extracellular vesicles;
- and
- b) comparing the level of the one or more small non-coding RNA to a reference level of the one or more small non-coding RNA, wherein an increased or decreased level of the one or more small non-coding RNA compared to the reference level is indicative of a disease state or a stage of development of a disease state.
A method for preparing an extracellular vesicle fraction enriched for neuron derived extracellular vesicles from a sample for analysing small non-coding RNA involved in a disease state, comprising:
- (a) providing a biological sample;
- (b) contacting the sample with a microtubule associated protein (MAP) binding agent to selectively bind extracellular vesicles expressing MAP;
- and
- (c) isolating the bound extracellular vesicles.
A method for the isolation of neuron derived extracellular from a blood sample, comprising:
- (a) contacting the sample with a microtubule associated protein (MAP) binding agent to selectively bind extracellular vesicles expressing MAP1B;
- and
- (b) isolating the bound extracellular vesicles.
A population of neuron derived extracellular vesicles, wherein the extracellular vesicles comprise one or more small non-coding RNA comprising a sequence selected from any one of SEQ ID NOs:
- 2, 6, 9, 21, or 29 to 51.
A population of neuron derived extracellular vesicles, wherein the extracellular vesicles comprise one or more small non-coding RNA comprising a sequence selected from any one of SEQ ID NOs:
- 30 to 32.
A kit for the isolation of neuron derived extracellular vesicles from a blood sample comprising:
- (a) a microtubule associated protein (MAP) binding agent;
- and
- (b) optionally, one or more agents selected a solid support, wash buffer, elution buffer.
Dependencies are inferred from the claim text.