Synthesis Method for Second Strand in DNB Paired-End Sequencing, Sequencing Method and Related Products
AssigneeMGI Tech Co., Ltd.
InventorsChongjun XU, Lan JIANG, Jin YANG, Meihua GONG
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Abstract
A synthesis method for a second strand in DNB paired-end sequencing, a sequencing method and a related product. The synthesis method for the second strand comprises: after a first-strand sequencing on the DNB is completed, performing a second-strand synthesis using a second-strand synthesis primer, wherein the second-strand synthesis primer is a primer capable of being anchored on the surface of a sequencing chip.
Claims12
A method for synthesizing a second-strand in a DNB paired-end sequencing, wherein the method comprises:
- upon completion of a first-strand sequencing on a DNB, synthesizing the second-strand using a second-strand synthesis primer, wherein the second-strand synthesis primer is an anchoring primer on a surface of a sequencing chip.
A sequencing chip, wherein a bridge molecule capable of being coupled with a primer having a terminal modification is modified on the surface of the sequencing chip, wherein the bridge molecule is any one selected from:
- NHS-PEG8-azido, SMCC, NHS-DBCO, and NHS-azido.
A kit, wherein the kit comprises any more of:
- Nb.BbvCI enzyme, human alkyladenine DNA glycosylase, endonuclease IV, endonuclease V, USER enzyme, formamide, a DBCO-bearing primer, an SH-bearing primer, an azido-bearing primer, an alkynyl-bearing primer, and Bst3.0 polymerase.
Dependencies are inferred from the claim text.