Adenoviral helper plasmid
AssigneeForge Biologics, Inc.
InventorsAngela M. Adsero, Brendan Chestnut, Linas Padegimas, David Dismuke
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Abstract
The present disclosure provides improved adenoviral helper plasmids for the production of recombinant adeno-associated viruses.
Claims53
An adenoviral helper plasmid comprising a nucleotide sequence encoding:
- (a) E2a protein;
- (b) an E4 region;
- and
- (c) a VA RNA region;
- wherein the adenoviral helper plasmid does not comprise a nucleotide sequence encoding one or more of:
- a Fiber protein or portion thereof;
- a L1-52/55K (Packaging Protein 3);
- and
- a Peripentonal Hexon-Associated Protein.
The adenoviral helper plasmid of any of the above claims, wherein the adenoviral helper plasmid comprises a resistance gene.
An adenoviral helper plasmid having 80% sequence identity to any one of SEQ ID NO:
- 80-105.
An adenoviral helper plasmid comprising an engineered E2A region comprising any one of nucleotide sequences SEQ ID NO:
- 106 or SEQ ID NO:
- 112.
An L4 trans plasmid comprising a nucleotide sequence encoding an engineered L4 region.
The method of 51 wherein, the L4 trans plasmid comprises a nucleotide sequence encoding L33K.
The method of 51 wherein, the producer cell a nucleotide sequence encoding an L4 region or portion thereof.
Dependencies are inferred from the claim text.