Method for preparing corona virus-like particles and vaccine comprising the same

Abstract

The present invention provides a method for producing coronavirus virus-like particles (VLPs) and a vaccine comprising the same. The method comprises: constructing a recombinant baculovirus carrying a coronavirus spike protein gene; transducing mosquito cells with the recombinant virus; and culturing the transduced cells in a serum-free medium to produce VLPs containing the coronavirus spike protein. Preferably, the mosquito cells are C6/36 cells, and the spike protein gene may be derived from SARS-COV, MERS-COV, or SARS-COV-2. Furthermore, the invention provides a vaccine containing the VLPs produced by the above method, which can be administered intranasally or by intramuscular injection.

Dependencies are inferred from the claim text.