Pesticidal Genes and Methods of Use

A nucleic acid molecule comprising:

• (a) a nucleotide sequence encoding a polypeptide comprising an amino acid sequence set forth in SEQ ID NO:
• 2;
• (b) a nucleotide sequence encoding a polypeptide comprising an amino acid sequence having at least the percent sequence identity set forth in Table 1 to an amino acid sequence set forth in SEQ ID NOs:
• 2, wherein the polypeptide has pesticidal activity;
• (c) a nucleotide sequence encoding a polypeptide comprising an amino acid sequence having at least 90% sequence identity to an amino acid sequence set forth in SEQ ID NO:
• 2, wherein the polypeptide has pesticidal activity;
• (d) a nucleotide sequence encoding a polypeptide comprising an amino acid sequence having at least 95% sequence identity to an amino acid sequence set forth in SEQ ID NO:
• 2, wherein the polypeptide has pesticidal activity;
• (e) a nucleotide sequence having at least 90% sequence identity to the nucleotide sequence set forth in SEQ ID NOs:
• 1 or 3, wherein the nucleotide sequence encodes a polypeptide having pesticidal activity;
• (f) a nucleotide sequence having at least 95% sequence identity to the nucleotide sequence set forth in SEQ ID NOs:
• 1 or 3, wherein the nucleotide sequence encodes a polypeptide having pesticidal activity;
• or
• (g) a nucleotide sequence comprising the nucleotide sequence set forth in SEQ ID NOs:
• 1 or 3, wherein the nucleotide sequence encodes a polypeptide having pesticidal activity.

A host cell comprising a nucleic acid molecule comprising:

• (a) a nucleotide sequence encoding a polypeptide comprising an amino acid sequence set forth in SEQ ID NO:
• 2;
• (b) a nucleotide sequence encoding a polypeptide comprising an amino acid sequence having at least the percent sequence identity set forth in Table 1 to an amino acid sequence set forth in SEQ ID NO:
• 2, wherein the polypeptide has pesticidal activity;
• (c) a nucleotide sequence encoding a polypeptide comprising an amino acid sequence having at least 90% sequence identity to an amino acid sequence set forth in SEQ ID NO:
• 2, wherein the polypeptide has pesticidal activity;
• (d) a nucleotide sequence encoding a polypeptide comprising an amino acid sequence having at least 95% sequence identity to an amino acid sequence set forth in SEQ ID NO:
• 2, wherein the polypeptide has pesticidal activity;
• (e) a nucleotide sequence having at least 90% sequence identity to the nucleotide sequence set forth in SEQ ID NOs:
• 1 or 3, wherein the nucleotide sequence encodes a polypeptide having pesticidal activity;
• (f) a nucleotide sequence having at least 95% sequence identity to the nucleotide sequence set forth in SEQ ID NOs:
• 1 or 3, wherein the nucleotide sequence encodes a polypeptide having pesticidal activity;
• or
• (g) a nucleotide sequence comprising the nucleotide sequence set forth in SEQ ID NOs:
• 1 or 3, wherein the nucleotide sequence encodes a polypeptide having pesticidal activity.

A DNA construct comprising a heterologous promoter operably linked to a nucleic acid sequence comprising:

• (a) a nucleotide sequence encoding a polypeptide comprising an amino acid sequence set forth in SEQ ID NO:
• 2;
• (b) a nucleotide sequence encoding a polypeptide comprising an amino acid sequence having at least the percent sequence identity set forth in Table 1 to an amino acid sequence set forth in SEQ ID NO:
• 2, wherein the polypeptide has pesticidal activity;
• (c) a nucleotide sequence encoding a polypeptide comprising an amino acid sequence having at least 90% sequence identity to an amino acid sequence set forth in SEQ ID NO:
• 2, wherein the polypeptide has pesticidal activity;
• (d) a nucleotide sequence encoding a polypeptide comprising an amino acid sequence having at least 95% sequence identity to an amino acid sequence set forth in SEQ ID NO:
• 2, wherein the polypeptide has pesticidal activity;
• (e) a nucleotide sequence having at least 90% sequence identity to the nucleotide sequence set forth in SEQ ID NOs:
• 1 or 3, wherein the nucleotide sequence encodes a polypeptide having pesticidal activity;
• (f) a nucleotide sequence having at least 95% sequence identity to the nucleotide sequence set forth in SEQ ID NOs:
• 1 or 3, wherein the nucleotide sequence encodes a polypeptide having pesticidal activity;
• or
• (g) a nucleotide sequence comprising the nucleotide sequence set forth in SEQ ID NOs:
• 1 or 3, wherein the nucleotide sequence encodes a polypeptide having pesticidal activity.

A formulation comprising a polypeptide, wherein the polypeptide comprises:

• (a) an amino acid sequence set forth in SEQ ID NO:
• 2;
• (b) an amino acid sequence having at least the percent sequence identity set forth in Table 1 to an amino acid sequence set forth in SEQ ID NO:
• 2, wherein the polypeptide has pesticidal activity;
• (c) an amino acid sequence having at least 90% sequence identity to an amino acid sequence set forth in SEQ ID NO:
• 2, wherein the polypeptide has pesticidal activity;
• or
• (d) an amino acid sequence having at least 95% sequence identity to an amino acid sequence set forth in SEQ ID NO:
• 2, wherein the polypeptide has pesticidal activity.

A plant having stably incorporated into its genome a DNA construct comprising a nucleic acid molecule, wherein said nucleic acid molecule comprises:

• (a) a nucleotide sequence that encodes a polypeptide comprising the amino acid sequence of any one of SEQ ID NO:
• 2;
• (b) a nucleotide sequence that encodes a polypeptide comprising an amino acid sequence having at least the percent sequence identity set forth in Table 1 to an amino acid sequence set forth in SEQ ID NO:
• 2, wherein the polypeptide has pesticidal activity;
• (c) a nucleotide sequence that encodes a polypeptide comprising an amino acid sequence having at least 90% sequence identity to an amino acid sequence set forth in SEQ ID NO:
• 2, wherein the polypeptide has pesticidal activity;
• (d) a nucleotide sequence that encodes a polypeptide comprising an amino acid sequence having at least 95% sequence identity to an amino acid sequence set forth in SEQ ID NO:
• 2, wherein the polypeptide has pesticidal activity;
• (e) a nucleotide sequence having at least 90% sequence identity to the nucleotide sequence set forth in SEQ ID NOs:
• 1 or 3, wherein the nucleotide sequence encodes a polypeptide having pesticidal activity;
• (f) a nucleotide sequence having at least 95% sequence identity to the nucleotide sequence set forth in SEQ ID NOs:
• 1 or 3, wherein the nucleotide sequence encodes a polypeptide having pesticidal activity;
• or
• (g) a nucleotide sequence comprising the nucleotide sequence set forth in SEQ ID NOs:
• 1 or 3, wherein the nucleotide sequence encodes a polypeptide having pesticidal activity.

A method for controlling insect pest damage to a plant, comprising expressing in a plant or cell thereof a nucleic acid molecule, wherein said nucleic acid molecule comprises

• (a) a nucleotide sequence that encodes a polypeptide comprising the amino acid sequence set forth in SEQ ID NO:
• 2;
• (b) a nucleotide sequence that encodes a polypeptide comprising an amino acid sequence having at least the percent sequence identity set forth in Table 1 to an amino acid sequence set forth in SEQ ID NO:
• 2, wherein the polypeptide has pesticidal activity;
• (c) a nucleotide sequence that encodes a polypeptide comprising an amino acid sequence having at least 90% sequence identity to an amino acid sequence set forth in SEQ ID NO:
• 2, wherein the polypeptide has pesticidal activity;
• (d) a nucleotide sequence that encodes a polypeptide comprising an amino acid sequence having at least 95% sequence identity to an amino acid sequence set forth in SEQ ID NO:
• 2, wherein the polypeptide has pesticidal activity;
• (e) a nucleotide sequence having at least 90% sequence identity to the nucleotide sequence set forth in SEQ ID NOs:
• 1 or 3, wherein the nucleotide sequence encodes a polypeptide having pesticidal activity;
• (f) a nucleotide sequence having at least 95% sequence identity to the nucleotide sequence set forth in SEQ ID NOs:
• 1 or 3, wherein the nucleotide sequence encodes a polypeptide having pesticidal activity;
• or
• (g) a nucleotide sequence comprising the nucleotide sequence set forth in SEQ ID NOs:
• 1 or 3, wherein the nucleotide sequence encodes a polypeptide having pesticidal activity.

A method for increasing yield in a plant comprising growing in a field a plant or seed thereof having stably incorporated into its genome a DNA construct comprising a promoter that drives expression in a plant operably linked to a nucleic acid molecule, wherein said nucleic acid molecule comprises:

• (a) a nucleotide sequence that encodes a polypeptide comprising the amino acid sequence set forth in SEQ ID NO:
• 2;
• (b) a nucleotide sequence that encodes a polypeptide comprising an amino acid sequence having at least the percent sequence identity set forth in Table 1 to an amino acid sequence set forth in SEQ ID NO:
• 2, wherein the polypeptide has pesticidal activity;
• (c) a nucleotide sequence that encodes a polypeptide comprising an amino acid sequence having at least 90% sequence identity to an amino acid sequence set forth in SEQ ID NO:
• 2, wherein the polypeptide has pesticidal activity;
• or
• (d) a nucleotide sequence that encodes a polypeptide comprising an amino acid sequence having at least 95% sequence identity to an amino acid sequence set forth in SEQ ID NO:
• 2, wherein the polypeptide has pesticidal activity;
• (e) a nucleotide sequence having at least 90% sequence identity to the nucleotide sequence set forth in SEQ ID NOs:
• 1 or 3, wherein the nucleotide sequence encodes a polypeptide having pesticidal activity;
• (f) a nucleotide sequence having at least 95% sequence identity to the nucleotide sequence set forth in SEQ ID NOs:
• 1 or 3, wherein the nucleotide sequence encodes a polypeptide having pesticidal activity;
• or
• (g) a nucleotide sequence comprising the nucleotide sequence set forth in SEQ ID NOs:
• 1 or 3, wherein the nucleotide sequence encodes a polypeptide having pesticidal activity.

A method of obtaining a polynucleotide that encodes an improved polypeptide having pesticidal activity, wherein the improved polypeptide has at least one improved property over SEQ ID NO:

• 2, said method comprising:
• (a) recombining a plurality of parental polynucleotides comprising any of SEQ ID NOs:
• 1 and 3 or an active variant or fragment thereof to produce a library of recombinant polynucleotides encoding recombinant pesticidal polypeptides;
• (b) screening the library to identify a recombinant polynucleotide that encodes an improved recombinant pesticidal polypeptide that has an enhanced property improved over the parental polynucleotide;
• (c) recovering the recombinant polynucleotide that encodes the improved recombinant pesticidal polypeptide identified in (b);
• and,
• (d) repeating steps (a), (b) and (c) using the recombinant polynucleotide recovered in step (c) as one of the plurality of parental polynucleotides in repeated step (a).