Tomato plant having improved whitefly resistance
AssigneeEnza Zaden Beheer B.V.
InventorsTeresa Montoro Ponsoda, Jan-Willem De Kraker, Juan David Cano Martinez, Marieke Ykema
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Abstract
The present invention relates to a tomato plant having improved insect resistance, more specifically whitefly or mite resistance, wherein the plant comprises a ASAT3 gene encoding for an acetyl-CoA-dependent acyltransferase enzyme and an AP2e gene encoding for a APETALA2 ethylene-responsive transcription factor. The present invention further relates to methods for providing a tomato plant having improved insect resistance and the use of a ASAT3 gene in combination with an AP2e gene for providing insect resistant tomato plants.
Claims22
A tomato plant having improved whitefly resistance, wherein said plant comprises a combination of an acetyl-CoA-dependent acyltransferase gene (ASAT3) encoding a cDNA sequence having at least 95% sequence identity with SEQ ID No. 1, and an APETALA2e ethylene-responsive transcription factor gene (AP2e) encoding a cDNA sequence having at least 95% sequence identity with SEQ ID No. 3, wherein said combination of ASAT3 and AP2e genes result in an increased acyl sugar content of one or more sugars selected from the group consisting of C39H66O15, C39H68O15, C38H66O15, C40H70O15, preferably all of said sugars, as compared to a tomato plant not comprising said combination of genes.
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A method for providing a tomato plant having improved whitefly resistance, the method comprises the steps of providing a whitefly susceptible tomato plant and mutating its genome comprising;
- providing a combination of an acetyl-CoA-dependent acyltransferase gene (ASAT3) encoding a cDNA sequence having at least 95% sequence identity with SEQ ID No. 1, and an APETALA2e ethylene-responsive transcription factor gene (AP2e) encoding a cDNA sequence having at least 95% sequence identity with SEQ ID No. 3, wherein said combination of ASAT3 and AP2e genes result in an increased of one or more of C39H66O15, C39H68O15, C38H66O15, C40H70O15 acyl sugar content as compared to a tomato plant not comprising said combination of genes.
A combination of an acetyl-CoA-dependent acyltransferase gene (ASAT3) encoding a cDNA sequence having at least 95% sequence identity with SEQ ID No. 1, and an APETALA2e ethylene-responsive transcription factor gene (AP2e) encoding a cDNA sequence having at least 95% sequence identity with SEQ ID No. 3 for providing insect resistance in tomato plants.
Dependencies are inferred from the claim text.