Genes altering soy plant flowering time and/or maturation and uses thereof
AssigneesInstitute of Crop Sciences, Chinese Academy of Agricultural Sciences, Syngenta Crop Protection AG
InventorsWensheng HOU, Yupeng CAI, Li CHEN, Shan YUAN, Qiang SU, Shi SUN, Tianfu HAN, Yang GAO
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Abstract
Compositions and methods for altering flowering and/or maturity time of soybean plant are provided. Compositions include isolated and recombinant polynucleotides encoding polypeptides, expression cassettes, host cells, plants, plant parts stably incorporating these polynucleotides. Methods and kits are provided for producing these plants via transgenic means, breeding or genomic editing approaches and identify plants having altered flowering and/or maturity time.
Claims73
A plant having a genomic modification, wherein the genomic modification comprises a knock out of one or more of the following genes:
- (i) GmCOL2a;
- (ii) GmCOL2b;
- (iii) GmFT5a;
- (iv) GmFT5b;
- or
- (v) GmFT4,
- wherein the plant has an altered flowering time and/or maturity time relative to a control plant not comprising the genomic modification.
A plant expressing both a mutant GmCOL2a polypeptide and a mutant GmCOL2b polypeptide,
- wherein mutant GmCOL2a polypeptide comprises:
- (a) an amino acid sequence that is at least 85% identical to SEQ ID NO:
- 20, or (b) an amino acid sequence as set forth in SEQ ID NO:
- 20, and
- wherein the mutant GmCOL2b polypeptide comprises an amino acid sequence that has at least 85% identity to SEQ ID NO:
- 26, or (b) an amino acid sequence as set forth in SEQ ID NO:
- 26.
A plant expressing both a mutant GmFT5a polypeptide and a mutant GmFT5b polypeptide,
- wherein mutant GmFT5a polypeptide comprises:
- (a) an amino acid sequence that is at least 85% identical to SEQ ID NO:
- 42, or (b) an amino acid sequence as set forth in SEQ ID NO:
- 42, and
- wherein the mutant GmFT5b polypeptide comprises an amino acid sequence that has at least 85% identity to SEQ ID NO:
- 38, or (b) an amino acid sequence as set forth in SEQ ID NO:
- 38.
A method of altering flowering time and/or maturity time in a soybean plant, the method comprising, editing in the genome of a soybean plant one or more of the following genes:
- (i) GmCOL2a
- (ii) GmCOL2b,
- (iii) GmFT5a,
- (iv) GmFT5b, or
- (v) GmFT4,
- thereby forming a modified soybean plant, wherein the modified soybean plant has a flowering time and/or maturity time that is altered relative to a control plant not comprising the editing in one or more of the genes.
The method of claim 60, wherein the delayed flowering is at least 2 days later, at least 4 days later, at least 5 days later, at least 6 days later, or at least 7 days later as compared to a control plant grown under LD conditions.
A plant comprising a genomic modification that results in decreased expression and/or activity of a polypeptide comprising:
- (a) an amino acid sequence comprising at least 85%, 90%, 91%, 92%, 93%, 94, 95%, 96%, 97%, 98%, or 99% identity to at least one of SEQ ID NOS:
- 7, 23, 28, 36, or 40, or
- (b) an amino acid sequence as set forth in at least one of SEQ ID NOS:
- 17, 23, 28, 36, or 40,
- wherein the modification is heterologous to the plant and the decreased expression and/or activity in the plant results in the plant having an altered flowering and/or maturity time compared to a control plant not comprising the genomic modification, and wherein the genomic modification is introduced via genome editing.
A modified soybean plant, or plant part thereof, comprising one or more non-naturally occurring mutant alleles at one or more loci, wherein the non-naturally occurring mutant allele is introduced via genomic modification using a site directed nuclease, wherein the one or more loci comprise GmFT4a, GmFT5a, GmFT5b, GmCOL2a, or GmCOL2b, and wherein the one or more mutant alleles result in an altered flowering and/or maturity time of the plant relative to a control plant not comprising the mutant allele.
Dependencies are inferred from the claim text.