Methods and compositions for reducing pod shatter in canola
AssigneePairwise Plants Services, Inc.
InventorsBrian Charles Wilding Crawford, Nathaniel Graham, Lolita George Mathew
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Abstract
This invention relates to compositions and methods for modifying SHATTERPROOF MADS-BOX (SHP) genes in canola plants, optionally to reduce pod shattering. The invention further relates to canola plants having reduced pod shatter produced using the methods and compositions of the invention.
Claims20
A canola plant or plant part thereof comprising at least one mutation in at least one endogenous SHATTERPROOF MADS-BOX (SHP) gene encoding a Shatterproof MADS-box transcription factor (SHP) polypeptide.
A method for producing a canola plant or part thereof comprising at least one cell having a mutated endogenous SHATTERPROOF MADS-BOX (SHP) gene, the method comprising
- contacting a target site in an endogenous SHP gene in the canola plant or plant part with a nuclease comprising a cleavage domain and a nucleic acid binding domain, wherein the nucleic acid binding domain binds to a target site in the endogenous SHP gene, wherein the endogenous SHP gene
- (a) comprises a nucleotide sequence having at least 80% sequence identity to any one of SEQ ID NOs:69, 70, 100, 101, 148, 149, 177, 178, 206, 207, 240 or 241;
- (b) comprises a region having at least 80% sequence identity to a nucleotide sequence of any one of SEQ ID NOs:72-96, 103-144, 151-173, 180-202, 209-236, 243-288 or 324-338;
- (c) encodes a SHP polypeptide having at least 80% sequence identity to any one of SEQ ID NOs:71, 102, 150, 179, 208, or 242;
- and/or
- (d) encodes a region of a SHP polypeptide having at least 80% sequence identity to any one of SEQ ID NOs:97-99, 145-147, 174-176, 203-205, 237-239 or 289-291, thereby producing the canola plant or part thereof comprising at least one cell having a mutation in the endogenous SHP gene.
Dependencies are inferred from the claim text.