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US 2026/0201369 A1Published application
Circrna expression construct
OwnerThomas HANSEN, Eoghan O'LEARYinventors
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Abstract
The present invention relates to a nucleic acid molecule encoding a circular RNA (circRNA). In another aspect the invention relates to a circRNA encoded by the nucleic acid and a vector and host cell comprising the nucleic acid. Further includes are methods of producing the circRNA.
Claims17
A nucleic acid molecule encoding a circular RNA (circRNA), wherein the nucleic acid molecule comprises:
- A) an expression cassette comprising:
- (i) a circRNA expression cassette comprising:
- (a) a nucleic acid comprising or consisting of a continuous or split open reading frame (ORF) encoding at least one protein, and
- (b) an internal ribosome entry site (IRES) operably linked to the ORF to direct translation of the ORF;
- (ii) a first backsplicing site that is positioned 5′ of the IRES;
- and
- (iii) a second backsplicing site that is positioned 3′ of the IRES;
- and optionally
- B) a first inverted repeat (IR) element positioned 5′ of the first backsplicing site and a second IR element positioned 3′ of the second backsplicing site;
- and/or
- a promoter operably linked to the expression cassette to direct expression of the expression cassette;
- wherein the IRES is positioned within the circRNA expression cassette in that:
- (I) the 5′ end of the IRES and the 3′ end of the first backsplicing site are separated by at least 50 nucleotides, preferably by at least 200 more preferably by at least 300 nucleotides, and
- (II) the 3′ end of the IRES and the 5′ end of the second backsplicing site are separated by at least 300 nucleotides, preferably by at least 350 nucleotides.
11 more elements
Dependencies are inferred from the claim text.