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US 2026/0201362 A1Published application
Robotic systems and methods for autonomous directed evolution of horizontally transferred nucleic acids
AssigneeMassachusetts Institute of Technology
InventorsKevin ESVELT, Erika Alden DEBENEDICTIS
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Abstract
The invention, in part, includes systems for conducting continuous directed evolution in a plurality of sample and methods of using the systems. The invention, in part also provides systems for evaluating the suitability of diverse engineered cells to accomplish directed evolution and methods of use of such systems.
Claims28
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A method for conducting discretized continuous directed evolution simultaneously in a plurality of samples, the method comprising:
- providing one or more receptacle in a plurality of receptacles, with one or more samples, wherein (i) each sample is in a separate receptacle in the plurality of receptacles;
- (ii) one or more independently selected receptacles in the plurality of receptacles comprises a cell;
- and (iii) one or more independently selected receptacles in the plurality of receptacles comprises one or more horizontally transferable nucleic acids;
- providing a liquid handling robot configured to perform liquid exchange in one or more receptacles in the plurality of receptacles;
- facilitating mixing in one or more receptacles in the plurality of receptacles;
- detecting at least a first detectable parameter in one or more of the samples in the plurality of samples;
- and
- modifying one or more of the samples in the plurality of receptacles in real-time based at least in part on the first detectable parameter of the one or more of the samples.
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Dependencies are inferred from the claim text.