Method for separating beta-xylosidase enzyme from an enzyme mixture
AssigneeIfp Energies Nouvelles
InventorsSimon ARRAGAIN, Fadhel BEN CHAABANE
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Abstract
The present invention relates to a process for separating beta-xylosidase enzymes from a mixture of enzymes comprising beta-xylosidase enzymes and other enzymes, such that the beta-xylosidase enzymes to be separated are devoid of a histidine group, and such that said beta-xylosidase enzymes are separated from the rest of the enzyme mixture by immobilized metal ion affinity chromatography IMAC.
Claims20
A process for separating beta-xylosidase enzymes (1) from a mixture of enzymes comprising beta-xylosidase enzymes and other enzymes, the process comprising:
- separating from the rest of the enzyme mixture by immobilized metal ion affinity chromatography (MAC), and wherein the beta-x vlosidase enzymes to be separated are devoid of a histidine group.
A method of enriching an enzyme cocktail produced by a microorganism with beta-xylosidase enzymes, comprising:
- separating from the enzyme cocktail by immobilized metal ion affinity chromatography (IMAC).
Dependencies are inferred from the claim text.