Method of inducing umbilical cord mesenchymal stem cells to differentiate insulin-like cells and an application thereof

A method of inducing umbilical cord mesenchymal stem cells to differentiate insulin-like cells, comprising the following steps:

• S1:
• preparing and obtaining umbilical cord mesenchymal stem cells from umbilical cord;
• S2:
• taking purified umbilical cord mesenchymal stem cells and add them to an α-MEM culture solution containing bFGF, EGF, and 5 wt % human platelet lysate, inducing differentiation of umbilical cord mesenchymal stem cells to nestin-positive progenitor cells NIP;
• S3:
• digesting the cells after the cells are fully spread in the culture bottle, adding an α-MEM culture solution containing glucose and 5 wt % human platelet lysate, and culturing for 2-4 days;
• S4:
• take cells, discard the culture supernatant, add them to an α-MEM culture solution containing ITS-A and 5 wt % human platelet lysate, culture for 3-5 days;
• S5:
• take cells, discard the culture supernatant, add them to an α-MEM culture solution containing 5 wt % human platelet lysate having ITS-A and nicotinamide, and culture for 2-4 days without changing the fluid during the period;
• S6:
• take cells, discard the culture supernatant, add them to an α-MEM culture solution containing 5 wt % human platelet lysate having β-cell regulator, IGF, ITS-A and nicotinamide, and culture for 2-5 days without changing the fluid during the period.