Reprogrammed smooth muscle cells and methods related thereto
AssigneesEmory University, Industry-Academic Cooperation Foundation, Yonsei University
InventorsYoung-sup YOON, Kyung Hee KIM, Ji Woong HAN, Cholomi JUNG, Shin-Jeong LEE
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Abstract
Provided herein are novel reprogrammed smooth muscle cells (rSMCs) and methods of making and using the cells for the treatment of ischemia. The rSMCs are produced by culturing a fibroblast with an all-trans-retinoic acid (ATRA) under conditions that produce the rSMC from the fibroblast, wherein the fibroblasts are genetically modified to overexpress myocardin. The rSMCs offer advantages over currently available regenerative vascular therapies by promoting vascular perfusion in a recipient subject. In particular, the rSMCs can increase neovascularization of both small and large vessels.
Claims19
A method of producing a reprogrammed smooth muscle cell, comprising culturing a fibroblast with an all-trans-retinoic acid (ATRA) under conditions that produce a reprogrammed smooth muscle cell from the fibroblast, wherein the fibroblasts are genetically modified to overexpress myocardin.
A reprogrammed smooth muscle cell comprising a heterologous nucleic acid encoding myocardin, wherein the reprogrammed smooth muscle cell co-expresses CNN1 and SMTN in a non-striated pattern.
Dependencies are inferred from the claim text.