In vitro cultivation method for antibody expressing cells
AssigneeRoche Diagnostics Operations, Inc.
InventorsJanis Brockhaus, Sebastian Buchenberg, Caroline Dorothea Hojer, Thomas Meier
Life of this patent
Estimating the term…
Abstract
The present invention relates to an in vitro method for cultivating one or more antibody expressing cell(s). The method comprises cultivating one or more antibody expressing cell(s) obtained from peripheral blood in the presence of IL-2, IL-21 and a non-cell surface presented CD40-stimulating agent and in the absence of feeder cells. Moreover, herein provided are methods for producing antibodies comprising the step of cultivating one or more antibody expressing cell(s) according to the method of the invention, a novel CD40-stimulating agent and uses therefrom as well as a cell culture medium.
Claims21
An in vitro method for cultivating one or more antibody expressing cell(s) obtained from peripheral blood, said method comprising:
- cultivating the one or more antibody expressing cell(s) in the presence of IL-2, IL-21 and a non-cell surface presented CD40-stimulating agent,
- wherein said cultivating is performed in the absence of feeder cells.
A non-cell surface bound CD40-ligand (CD40-L) comprising (i) an CD40-L domain comprising or consisting of the amino acid sequence of SEQ ID NO:
- 1, 2, 3, 4, 5 or 6 or a variant thereof with CD40-L activity having at least 75%, at least 80%, at least 85%, at least 90%, at least 95% or at least 99% sequence identity thereto and (ii) optionally a multimerization domain.
(canceled)
Dependencies are inferred from the claim text.