Recombinant escherichia coli for de novo biosynthesis of anisic acid and production method and application thereof

A recombinant Escherichia coli strain for de novo biosynthesis of p-anisic acid, wherein at least one of the following improvements has been made to a starting strain of E. coli:

• (1) overexpression of O-methyltransferase PSA-OMT1 from Pleurotus sapidus;
• overexpression of UbiC, a chorismic acid lyase derived from E. coli MG1655 or Providencia rustigianii;
• (2) on the basis of (1), overexpression of a feedback inhibition mutant AroGD146N derived from 3-deoxy-D-arabino-heptulosonate 7-phosphate, (DAHP) synthase of E. coli MG1655;
• (3) on the basis of (2), overexpression of PpsA derived from phosphoenolpyruvate, (PEP) synthase, and knockout of pykF gene encoding a pyruvate kinase I;
• a nucleotide sequence of codon optimized O-methyltransferase PSA-OMT1 is shown in SEQ ID No.:
• 1;
• a nucleotide sequence of the chorismic acid lyase UbiC derived from E. coli MG1655 is shown in SEQ ID No.:
• 2;
• a nucleotide sequence of codon optimized chorismic acid lyase UbiC derived from Providencia rustigianii is shown in SEQ ID No.:
• 3;
• a nucleotide sequence of the feedback inhibition mutant AroGD146N of DAHP synthase is shown in SEQ ID No.:
• 4;
• a nucleotide sequence of the PEP synthase PpsA is shown in SEQ ID No.:
• 5.