Genetically modified udp-n-acetylgalactosamine producing cells

Abstract

The present disclosure relates to a genetically modified cell capable of producing UDP-N-acetylglucosamine (UDP-GlcNAc), wherein the mRNA stability of the native glucosamine-6-phopshate synthase (glmS) is enhanced by modifying said cell such that it has decreased or total loss of function of RNase adapter protein RapZ (yhbJ). In particular, the present disclosure relates to said cells that are likewise capable of producing one or more N-acetylated- and/or sialylated molecules, such as N-acetylated- and/or sialylated human milk oligosaccharides (HMOs). The present disclosure further relates to a method for producing one or more N-acetylated- and/or sialylated human milk oligosaccharides (HMO) making use of such cells.

Dependencies are inferred from the claim text.