A rotating tissue culture insert for the position inside a rotatable roller bottle partially filled with a liquid for the cultivation of cells
AssigneeUniversitäT ZüRich
InventorsEmanuela S. FIORETTA, Nikolaos POULIS, Maximilian Y. EMMERT, Simon P. HOERSTRUP
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Abstract
A rotating tissue culture insert for positioning inside a rotatable roller bottle partially filled with a liquid for the cultivation of cells includes a scaffold provided for the cultivation of cells, an axle body, and at least two protrusions extending in radial direction from the axle body. The protrusions define wheels of the rotating tissue culture insert with a runway that is in contact with the surface of a roller bottle when the roller bottle is in a lying position.
Claims16
1-15. (canceled)
A rotating tissue culture insert comprising:
- a scaffold configured to cultivate cells;
- an axle body;
- and
- at least two protrusions extending in a radial direction from the axle body,
- wherein the rotating tissue culture insert is configured to be positioned inside a rotatable roller bottle partially filled with a liquid to cultivate the cells, and
- wherein the at least two protrusions are wheels of the rotating tissue culture insert with a runway that is in contact with a surface of a roller bottle when the roller bottle is in a lying position.
A method for producing of an implant comprising, the method comprising:
- a. providing a roller bottle comprising a liquid for cultivating cells and a rotating tissue culture insert, wherein the rotating tissue culture insert comprises a scaffold configured to cultivate the cells, an axle body, and at least two protrusions extending in a radial direction from the axle body, wherein the rotating tissue culture insert is positioned inside the roller bottle, wherein the at least two protrusions are wheels of the rotating tissue culture insert with a runway that is in contact with a surface of a roller bottle when the roller bottle is in a lying position such that a fill level of the roller bottle is parallel to a longitudinal axis of the bottle, wherein the rotating tissue culture insert further comprises a scaffold holder attaching the scaffold to the rotating tissue culture insert;
- b. rotating the roller bottle, wherein the scaffold is distanced towards a wall of the roller bottle, wherein the rotating tissue culture insert rotates in the roller bottle, and wherein a tissue is formed at a surface of the scaffold during the rotation of the roller bottle;
- and
- c. removing an arrangement comprising at least the tissue and the scaffold holder from the axle body of the rotating tissue culture insert, wherein the arrangement is an integral part of the implant.
Dependencies are inferred from the claim text.