Systems and methods for cell culturing
AssigneeFlaskworks, LLC
InventorsShashi K. Murthy, Andrew Kozbial
Life of this patent
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Abstract
Cell culture systems and methods provide improved immunotherapeutic product manufacturing with greater scalability, flexibility, and automation. Cell culture systems are configured with interchangeable cartridges, allowing versatility and scalability. Systems are configured to have multiple connected cell culture chambers, which allows parallel processing of different types of cells. Gas-impermeable cell culture chambers and methods for generating cells in closed systems prevent contamination and user error. Methods for recycling cell culture medium provide additional efficiencies.
Claims21
-20. (canceled)
A method for culturing cells, the method comprising:
- providing a cell culture system comprising an inlet, an outlet, at least one perfusion fluid reservoir, at least one waste fluid reservoir, at least one pump, one or more sensors configured to measure one or more parameters, and a control system operably associated with the cell culture chamber;
- flowing perfusion fluid comprising a cell culture medium into the cell culture chamber;
- removing used cell culture medium from the cell culture chamber;
- receiving data from the one or more sensors;
- assessing one or more parameters of the used cell culture medium based on the data received from the one or more sensors;
- and
- redirecting one or more portions of the used cell culture medium from the waste fluid reservoir back to the cell culture chamber based on whether the assessed one or more parameters meets a predetermined threshold
The method of claim 1, wherein a rate of redirecting the used cell culture medium from the waste reservoir is based, at least in part, on at least one of required gas exchange for cell culturing and required nutrient supply for cell culturing.
Dependencies are inferred from the claim text.