Cell culture system and cell culture method
AssigneesFundació Institut De Bioenginyeria De Catalunya, Institució Catalana De Recerca I Estudis AvançAts, Fundació Institut Hospital Del Mar D'Investigacions
InventorsAnna LABERNADIE, Xavier TREPAT GUIXER, Alexandre CALON
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Abstract
A cell culture system (1) comprising a body of the device (2) separating a first zone (3) and a second zone (4) and comprising at least one microchannel (5) fluidly communicating the first zone (3) with the second zone (4). A cell culture plate or a well plate (18) comprising at least one cell culture system (1). and a method for cell culturing using the cell culture system (1), comprising seeding a first cell type in the first zone (3) and seeding a second cell type on top of the first cell type. This method may further comprise the application of a treatment in the second zone (4) so that it passes to the first zone (3) through the at least one microchannel (5).
Claims13
A cell culture system (1) suitable for studying cell-cell interactions and for drug screening comprising:
- a body of the device (2) separating a first zone (3) and a second zone (4), said body of the device (2) comprises at least one microchannel (5), arranged in the bottom surface of the body of the device (2), and fluidly communicating the first zone (3) with the second zone (4) and the at least one microchannel (5) comprises a first communicating end (6) in communication with the first zone (3) and a second communicating end (7) in communication with the second zone (4);
- wherein the at least one microchannel (5) comprises a width (11) between 10 and 200 microns, height (12) between 5 and 50 microns, and length (13) between 200 microns and 2 millimeters;
- and
- wherein the first zone (3) comprises an inlet chamber (15) with a diameter of at least 4 millimeters.
Dependencies are inferred from the claim text.