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US 2026/0201292 A1Published application
Integrated system for 3d tissue culture
AssigneeValo Health, Inc.
InventorsRoozbeh Aschar-Sobbi, Maw-Lin Kuo, Roger Stenerson, Winston Sun, Dickshitha Thyagharajon, Ya Kevin Thao, Chris Todd, Brian Wilfley
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Abstract
Embodiments described herein relate generally to devices, apparatuses, and systems with embedded electrodes for growing, maintaining, and/or using 3D tissues in vitro. The devices, apparatuses, and systems described herein can provide scalable, automated tissue stimulation.
Claims19
An apparatus, comprising:
- a memory;
- and
- a processor operatively coupled to the memory, the processor configured to:
- receive an image of a tissue growing around a wire within a well from cells seeded within the well,
- define a first binary image based on comparing each pixel from a plurality of pixels in the image to a pixel threshold,
- detect a first edge and a second edge of the wire within the first binary image to define a second binary image representing the first edge and the second edge,
- calculate a midpoint between the first edge and the second edge for each row from a plurality of rows in the second binary image to produce a set of midpoints,
- calculate a quadratic equation that fits the set of midpoints based on a polynomial regression of the set of midpoints, and
- identify a point of deflection in the wire based on the quadratic equation.
6 more elements
A computer-implemented method comprising:
- receiving an image of a tissue growing around a wire within a well from cells seeded within the well;
- defining a first binary image based on comparing each pixel from a plurality of pixels in the image to a pixel threshold;
- detecting a first edge and a second edge of the wire within the first binary image to define a second binary image representing the first edge and the second edge;
- calculating a midpoint between the first edge and the second edge for each row from a plurality of rows in the second binary image to produce a set of midpoints;
- calculating a quadratic equation that fits the set of midpoints based on a polynomial regression of the set of midpoints;
- and
- identifying a point of deflection in the wire based on the quadratic equation.
4 more elements
A non-transitory processor-readable medium storing code representing instructions to be executed by a processor, the instructions comprising code to cause the processor to:
- receive an image of a tissue growing around a wire within a well from cells seeded within the well;
- define a first binary image based on comparing each pixel from a plurality of pixels in the image to a pixel threshold;
- detect a first edge and a second edge of the wire within the first binary image to define a second binary image representing the first edge and the second edge;
- calculate a midpoint between the first edge and the second edge for each row from a plurality of rows in the second binary image to produce a set of midpoints;
- calculate a quadratic equation that fits the set of midpoints based on a polynomial regression of the set of midpoints;
- and
- identify a point of deflection in the wire based on the quadratic equation.
4 more elements
Dependencies are inferred from the claim text.