Lipid comprising docosapentaenoic acid

A population of at least 2,000,000 transgenic Brassica napus plants, or seed obtained from the transgenic Brassica napus plants, each plant comprising

• i) lipid in its seed, the lipid comprising fatty acids in an esterified form, and
• ii) exogenous polynucleotides encoding a Δ12-desaturase having a sequence of amino acids provided as SEQ ID NO:
• 4, a ω3-desaturase having a sequence of amino acids provided as SEQ ID NO:
• 6, a Δ6-desaturase having a sequence of amino acids provided as SEQ ID NO:
• 9, a Δ5-desaturase having a sequence of amino acids provided as SEQ ID NO:
• 20, a Δ6-elongase having a sequence of amino acids provided as SEQ ID NO:
• 16 and a Δ5-elongase having a sequence of amino acids provided as SEQ ID NO:
• 25, wherein the plants are transgenic for the exogenous polynucleotides,
• wherein each polynucleotide is operably linked to one or more seed-specific promoters that are capable of directing expression of said polynucleotides in developing seeds of the plants, wherein the fatty acids of the lipid comprise oleic acid, palmitic acid, ω6 fatty acids comprising linoleic acid (LA), ω3 fatty acids comprising α-linolenic acid (ALA), docosapentaenoic acid (DPA), stearidonic acid (SDA), eicosapentaenoic acid (EPA) and eicosatetraenoic acid (ETA), wherein the level of palmitic acid in the total fatty acid content of the lipid is between 2% and 16% by weight, wherein the level of myristic acid (C14:0) in the total fatty acid content of the lipid, if present, is less than 1% by weight, wherein at least 70% of the DPA esterified in the form of TAG is in the sn-1 or sn-3 position of the TAG.