Loading patent…
OwnerDaniel Vincent ZURAWSKI, Mariel Giselle ESCATTE +1inventors
InventorsDaniel Vincent ZURAWSKI, Mariel Giselle ESCATTE, Yoann Stephane LE BRETON
Estimating the term…
Two surface proteins of A. baumannii, which are required for iron transport of the bacteria, were identified: BauA, which is a siderophore receptor, and OmpW2, which are porins. Amino acid- and DNA sequences of monoclonal antibodies that bind to BauA and OmpW2 antigens were identified. These monoclonal antibodies were able to reduce bacterial growth and biofilm formation, and when they were applied together, they work in synergy. It was also demonstrated that these antibodies prevented infection in three separate animal models of infection. Thus, the invention provides pharmaceutically applicable compositions and methods of use thereof as well as a kit for outdoor use, based on these mouse monoclonal antibodies in order to develop human monoclonal antibodies against BauA and/or OmpW2 of A. baumannii as surface targets. These antibodies block their function and render the bacteria avirulent and bacteriostatic so it can be killed by the immune system.
A monoclonal antibody against BauA of Acinetobacter baumannii or an antigen-binding fragment thereof, comprising;
(canceled)
(canceled)
(canceled)
A monoclonal antibody against OmpW2 of Acinetobacter baumannii or an antigen-binding fragment thereof, comprising;
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
A host cell comprising at least one nucleic acid molecule selected from SEQ ID NO:1, 6, 11, 21, 16, 26, or a nucleic acid sequence at least 90% identical to the SEQ ID NO:1, 6, 11, 21, 26 or 16, and/or a recombinant vector comprising at least one nucleic acid molecule selected from SEQ ID NO:1, 6, 11, 21, 16, 26, or a nucleic acid sequence at least 90% identical to the SEQ ID NO:1, 6, 11, 21, 26, or 16.
(canceled)
(canceled)
A composition for prophylactic or therapeutic treatment of A. baumannii infection, comprising a monoclonal antibody against BauA of Acinetobacter baumannii or an antigen-binding fragment thereof, comprising:
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
Dependencies are inferred from the claim text.