Methods for expression of fusion-free bovine ultralong cdr3 scaffold
AssigneePelican Technology Holdings, Inc.
InventorsRussell COLEMAN, Yinghui LEE
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Abstract
The present invention provides methods for producing a recombinant ultralong CDR3 knob peptide. Described methods comprise: culturing a Pseudomonadales host cell in a culture medium and expressing the recombinant ultralong CDR3 knob peptide in the periplasm of the Pseudomonadales host cell from an expression construct comprising a nucleic acid encoding the recombinant ultralong CDR3 knob peptide directly and operably linked to a periplasmic secretion leader; wherein the recombinant ultralong CDR3 knob peptide is produced by secretion into the periplasm of the Pseudomonadales host cell, and wherein the produced recombinant ultralong CDR3 knob peptide is present in the periplasm in soluble form, active form, or both.
Claims40
A method for producing a recombinant ultralong CDR3 knob peptide, the method comprising:
- culturing a Pseudomonadales host cell in a culture medium and expressing the recombinant ultralong CDR3 knob peptide in the periplasm of the Pseudomonadales host cell from an expression construct comprising a nucleic acid encoding the recombinant ultralong CDR3 knob peptide directly and operably linked to a periplasmic secretion leader;
- wherein the recombinant ultralong CDR3 knob peptide is produced by secretion into the periplasm of the Pseudomonadales host cell, and wherein the secreted recombinant ultralong CDR3 knob peptide is present in soluble form, active form, and/or in properly processed form.
Dependencies are inferred from the claim text.