Engineered platelet-derived growth factor variants
AssigneeMultus Biotechnology Limited
InventorsKevin PAN, Seong Hyun Brandon MA, Morgane VALLES, George Mackford TAYLOR
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Abstract
The invention relates to improved platelet-derived growth factor (PDGF) polypeptide variants, in particular polypeptides comprising a sequence of any one of SEQ ID NOs: 2-4 or a sequence with at least 80% similarity thereto, wherein the PDGF polypeptide exhibits increased stability, bioactivity and/or solubility compared to the wild-type PDGFB polypeptide of SEQ ID NO: 1. The invention further provides nucleic acid sequences encoding a PDGF polypeptide of the invention, and a method of growing an animal cell comprising cultivating the animal cell in a culture medium comprising a PDGF polypeptide of the invention is also provided.
Claims17
A platelet-derived growth factor (PDGF) polypeptide comprising at least one modification selected from the group consisting of an amino acid substitution, an amino acid deletion, an amino acid insertion, and a combinations thereof relative to the sequence set forth in SEQ ID NO:
- 1, wherein the modified PDGF polypeptide exhibits increased stability, solubility and/or bioactivity compared to the PDGFB polypeptide of SEQ ID NO:
- 1.
(canceled)
Dependencies are inferred from the claim text.