Methods for incubating and analyzing a cell in a compartment of a fluidic device
AssigneeCellanome, Inc.
InventorsTarun Kumar KHURANA, Ali AGAH, Yir-Shyuan WU, Filiz Gorpe YASAR, Vernon A. NORVIEL
Life of this patent
Estimating the term…
Abstract
Described herein are systems and methods for analyzing cells, including a method comprising: (a) forming a compartment within a fluid, wherein the fluid comprises a plurality of cells, wherein the compartment encloses a cell of the plurality of cells, and wherein the compartment physically separates the cell from an additional cell of the plurality of cells; (b) incubating the cell for about 1 minute to about 10 hours, wherein the cell is inside of the compartment during the incubating; and (c) performing an assay within the compartment, thereby analyzing the cell.
Claims27
(canceled)
A method, comprising:
- (a) introducing a plurality of live cells into a fluidic device such that (i) a first individual live cell of the plurality of live cells is located in a first discrete area of the fluidic device and (ii) a second individual live cell of the plurality of live cells is located in a second discrete area of the fluidic device, wherein the first discrete area is different from the second discrete area;
- (b) measuring an interaction between the first individual live cell and the second individual live cell;
- and
- (c) in the first discrete area, releasing nucleic acids from the first individual live cell.
A method, comprising:
- (a) introducing a plurality of live cells into a fluidic device such that (i) a first individual live cell of the plurality of live cells is located in a first discrete area of the fluidic device and (ii) a second individual live cell of the plurality of live cell is located in a second discrete area of the fluidic device, wherein the first discrete area is different from the second discrete area;
- (b) measuring an interaction between the first individual live cell and the second individual live cell;
- and
- (c) in the first discrete area, sequencing nucleic acids of the first individual live cell.
Dependencies are inferred from the claim text.