Crosslinked streptavidin chromatographic materials and methods of use thereof
AssigneeWaters Technologies Corporation
InventorsBeatrice Muriithi, Yeliz Tunc Sarisozen, Martin Gilar, Kevin Wyndham
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Abstract
Disclosed herein are chromatographic materials comprising crosslinked streptavidin molecules. The crosslinked streptavidin chromatographic materials may be used to prepare affinity chromatographic materials using biotinylated affinity agents, such as a biotinylated antibody, biotinylated antigen-binding fragment, or biotinylated oligonucleotide. Due to the crosslinked nature of the streptavidin molecules, the materials provided herein have reduced to no streptavidin leachate, resulting in improved performance.
Claims22
A method of generating crosslinked streptavidin particles, the method comprising:
- a) contacting a plurality of streptavidin molecules with a crosslinker to generate a plurality of crosslinked streptavidin molecules;
- and
- b) contacting the plurality of crosslinked streptavidin molecules with a plurality of nonporous particles to generate a plurality of crosslinked streptavidin-conjugated nonporous particles.
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Dependencies are inferred from the claim text.