Method and device for enriching and detecting microorganisms in a biological sample
AssigneeMicronbrane Medical Co., Ltd.
InventorsYung Chang, Mengchu Wu, Jheng-Fong Jhong, Yan-Wen Chen, Hau Hung
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Abstract
Disclosed are a method and system for enriching and detecting microorganisms in a biological sample. The method allows the biological sample to be filtered through a polymer-modified substrate. The polymer-modified substrate is highly specific in capturing or separating human-derived nucleated cells, and allows the microorganisms to penetrate through it. During the process, a high level of microorganisms (bacteria, mycoplasmas, fungi, viruses, spores etc.) can be enriched in the sample and thus the interference from nucleated cells such as leukocytes can be reduced.
Claims19
A method for enriching and detecting microorganisms in a biological sample, comprising the following steps:
- a) collecting the biological sample including microorganisms and human-derived nucleated cells;
- b) filtering the biological sample through a polymer-modified substrate to obtain a filtrate, wherein the human-derived nucleated cells in the biological sample are captured or separated by the polymer-modified substrate and the microorganisms in the biological sample pass or flow through the polymer-modified substrate into the filtrate;
- and
- c) detecting the microorganisms present in the filtrate, wherein:
- the human-derived nucleated cells include one or more of erythroblasts, leukocytes and cancer cells;
- and
- the polymer is prepared by the polymerization of one or more monomers having the structure of formula (1):
- wherein R1 is independently selected from the group consisting of hydrogen, methyl, ethyl, hydroxyl, C1-12 alkyl, phenyl;
- R2 is independently selected from the group consisting of hydrogen, methyl, ethyl, C1-6 alkyl, amino, phenyl;
- and n is an integer of 1 to 5.
Dependencies are inferred from the claim text.