Temperature-sensitive degradable gel for treating intrauterine adhesion and preparation method thereof

A preparation method of a temperature-sensitive degradable gel for treating intrauterine adhesion, comprising the following steps:

• S1:
• preparation of an amygdalin-loaded polymer
• preparing a soybean protein isolate solution and a carboxymethyl konjac glucomannan solution from soybean protein isolates and carboxymethyl konjac glucomannan, respectively, adding amygdalin to the soybean protein isolate solution, and then mixing with the carboxymethyl konjac glucomannan solution for polymerization to obtain the amygdalin-loaded polymer;
• S2:
• preparation of hydroxyproline-modified chitosan
• dissolving and activating hydroxyproline, then adding to an acetic acid solution of chitosan, followed by adding triethylamine and performing stirring reaction to obtain the hydroxyproline-modified chitosan;
• and
• S3:
• mixed preparation of the temperature-sensitive degradable gel
• dissolving polygalacturonic acid, sodium β-glycerophosphate, and the hydroxyproline-modified chitosan, respectively, stirring and mixing same in an ice-water bath, then adding autologous platelet-rich plasma and the amygdalin-loaded polymer, and performing homogeneous mixing to obtain the temperature-sensitive degradable gel;
• preparation of the autologous platelet-rich plasma comprising the following steps:
• first, collecting peripheral blood of a patient for anticoagulation treatment, and then performing centrifugation to separate the peripheral blood into an upper layer, a middle layer, and a lower layer;
• subsequently, aspirating the upper layer and the middle layer for secondary centrifugation;
• discarding supernatant, then adding thrombin and 8-10 wt % of a calcium chloride solution to a remaining portion to obtain the autologous platelet-rich plasma, wherein a volume percentage content of the calcium chloride solution in the autologous platelet-rich plasma is 8-12%, and a content of the thrombin therein is 15-25 U/mL.