Xten conjugate compositions and methods of making same
AssigneeAmunix Pharmaceuticals, Inc.
InventorsSheng DING, Chen GU, Bryant MCLAUGHLIN, Vladimir PODUST, Volker SCHELLENBERGER, Bee-Cheng SIM, Chia-Wei WANG
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Abstract
The present invention relates to extended recombinant polypeptide (XTEN) compositions, conjugate compositions comprising XTEN and XTEN linked to cross-linkers useful for conjugation to pharmacologically active payloads, methods of making highly purified XTEN, methods of making XTEN-linker and XTEN-payload conjugates, and methods of using the XTEN-cross-linker and XTEN-payload compositions.
Claims19
A polypeptide that comprises a helper sequence (HS) fused to the N-terminus of an extended recombinant polypeptide (XTEN), wherein the HS comprises a sequence that has at least 90% sequence identity to a sequence in Table 10.
A polypeptide comprising the sequence of SEQ ID NO:
- 517.
A method of selecting a combination of payloads linked to XTEN as a therapeutic agent, the method comprising
- a. providing a library of XTENs comprising a plurality of XTEN sequences wherein each of said XTEN sequences is conjugated to at least a first payload and at least a second payload which is different from the first payload;
- b. from said library, selecting an XTEN sequence as the therapeutic agent if it exhibits an improved in vitro or in vivo parameter as compared to that of (1) an XTEN sequence conjugated to the first payload alone;
- and (2) an XTEN sequence conjugated to the second payload alone.
Dependencies are inferred from the claim text.