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US 2026/0199386 A1Published application
Synthetic single-stranded dna molecules and methods of producing and using the same
AssigneeGeneration Bio Co.
InventorsRussell Monds, Kelly Ann Miller, Anthony Rohit Dawson, Daniel Jason Blackstock
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Abstract
The present application discloses modified single-stranded DNA molecules, as well as their cell-free methods of synthesis and their use as therapeutic agents.
Claims129
A method for producing a linear, single-stranded DNA (ssDNA) molecule comprising at least one nucleic acid sequence of interest flanked by at least one stem-loop structure comprising at least one stem and at least one loop at the 3′ end, the method comprising the sequential steps of:
- (a) contacting a double-stranded, closed-ended DNA (ceDNA) molecule comprising the at least one nucleic acid sequence of interest with an endonuclease;
- (b) contacting the double-stranded ceDNA with an exonuclease,
- thereby producing the linear, ssDNA molecule.
Dependencies are inferred from the claim text.