US 2026/0199238 A1 · LIPID PARTICLES WITH COFUSOGENS AND METHODS OF PRODUCING AND USING THE SAME

Abstract

Provided herein are lipid particles containing at least two attachment proteins derived from a paramyxovirus envelope attachment protein and at least one paramyxovirus fusion (F) protein. Also provided are lipid particles are viral vectors, such as lentiviral vectors or lentiviral-like particles. Also provided are producer cells and compositions containing such lipid particles and methods of making and using the lipid particles.

Claims180

Dependent claims

A lipid particle, comprising:

(a) a first retargeted attachment protein comprising (i) a first paramyxovirus envelope attachment protein;
and (ii) a first targeting moiety directed to a first target molecule expressed on the surface of a target cell,
(b) a second retargeted attachment protein comprising (i) a second paramyxovirus envelope attachment protein;
and (ii) a second targeting moiety directed to a second target molecule expressed on the surface of a target cell;
and
(c) at least one paramyxovirus fusion (F) protein;
wherein each of the first targeting moiety and the second targeting moiety are independently selected from the group consisting of an antibody or antigen-binding fragment, an engineered binding domain, a nanobody, a DARPin, an Aptamer, an Affimer, an Affibody, a Knottin, an Avimer, a Monobody, an Anticalin, a Fynomer, and a targeting peptide;
and
wherein the protein in (a), (b) and (c) are exposed on the outside of the lipid bilayer.

6 more elements

A lipid particle, comprising:

(a) a first retargeted attachment protein comprising (i) a first paramyxovirus envelope attachment protein;
and (ii) a first targeting moiety directed to CD133;
and
(b) a second retargeted attachment protein comprising (i) a second paramyxovirus envelope attachment protein;
and (ii) a second targeting moiety directed to CD133;
(c) a third paramyxovirus envelope attachment protein that is a variant paramyxovirus envelope attachment protein comprising one or more mutations that reduces the native tropism relative to the wild-type paramyxovirus envelope attachment protein not comprising the one or more mutations;
and
(d) at least one paramyxovirus fusion (F) protein;
wherein:
the first and second targeting moiety bind to distinct epitopes on CD133;
and
the protein in (a), (b), (c), and (d) are exposed on the outside of the lipid bilayer.

9 more elements

Dependencies are inferred from the claim text.

Lipid particles with cofusogens and methods of producing and using the same

Life of this patent

Lipid particles with cofusogens and methods of producing and using the same

Life of this patent

Abstract

Claims180