Humanized chimeras for the prospective assessment of cell addition and replacement therapies
AssigneesUniversity of Rochester, University of Copenhagen
InventorsSteven A. Goldman, Ricardo Da Costa Barbedo Vieira
Life of this patent
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Abstract
A chimeric non-human mammal disease model, wherein (1) at least 30% of all the glial cells in the corpus callosum of the chimeric non-human mammal are human glial cells, and/or (2) at least 5% of all of the glial cells in the white matter of the brain and/or brain stem of the chimeric non-human mammal are human glial cells, and wherein the human glial cells comprise a combination of a first group of human glial cells tagged with a first label and a second group of human glial cells tagged with a second label that is distinguishable from the first label.
Claims20
A chimeric non-human mammal, wherein (1) at least 30% of all the glial cells in the corpus callosum of the chimeric non-human mammal are human glial cells, and/or (2) at least 5% of all of the glial cells in the white matter of the brain and/or brain stem of the chimeric non-human mammal are human glial cells, and
- wherein the human glial cells comprise a combination of human disease-specific glial cells and healthy human glial cells, wherein the human disease-specific glial cells are tagged with a first detectable label, and wherein the healthy human glial cells are tagged with a second detectable label that is distinguishable from the first detectable label.
A chimeric non-human mammal, wherein (1) at least 30% of all the glial cells in the corpus callosum of the chimeric non-human mammal are human glial cells, and/or (2) at least 5% of all of the glial cells in the white matter of the brain and/or brain stem of the chimeric non-human mammal are human glial cells, and
- wherein the human glial cells comprise a first group of healthy human glial cells tagged with a first detectable label and a second group of healthy human glial cells tagged with a second detectable label that is distinguishable from the first detectable label.
A method for producing a chimeric non-human mammal comprising human glial cells, the method comprises the steps of:
- introducing a first population of human glial progenitor cells into the brain and/or brain stem of a non-human mammal, wherein the first population of human glial progenitor cells are tagged with a first detectable label;
- introducing a second population of human glial progenitor cells into the brain and/or brain stem of a non-human mammal, wherein the second population of human glial progenitor cells are tagged with a second detectable label that is distinguishable from the first detectable label;
- and
- recovering, as a result of said introducing, a chimeric non-human mammal with human glial cells at least partially replacing native glial cells in the brain or brain stem, wherein (1) at least 30% of all the glial cells in the corpus callosum of the chimeric non-human mammal are human glial cells, and/or (2) at least 5% of all of the glial cells in the white matter of the brain and/or brain stem of the chimeric non-human mammal are human glial cells.
Dependencies are inferred from the claim text.