Biomarkers in ex vivo lung perfusion (evlp) perfusate

A method for the early detection of donor lungs that will be declined at the end of the EVLP process comprising:

• a) obtaining one or more test EVLP perfusate samples of a perfusion EVLP solution collected during perfusion of the donor lung;
• b) determining one or more parameter values related to a level of one or more biomarkers selected from:
• IL-6 and sTREM-1;
• IL-6 and sTNFR1;
• and
• IL-6, sTREM-1, and sTNFR1;
• c) optionally generating a transplant suitability score for the donor lung based on the one or more parameter values;
• d) comparing the one or more parameter values or optionally the transplant suitability score for the donor lung with a control or cut-off level, and
• e) continuing perfusion if the one or more parameter values or transplant suitability score indicates that the donor lung is suitable for transplantation and discontinuing perfusion if the one or more parameter values or the transplant suitability score indicates that the donor lung will be declined for transplantation.

A method of selecting a candidate donor lung for transplant, the method comprising:

• a) obtaining one or more test EVLP perfusate samples of a perfusion solution collected during perfusion of the donor lung:
• b) measuring one or more parameter values related to a level of one or more biomarkers selected from IL-6 and sTREM-1;
• IL-6 and sTNFR1;
• and IL-6 and sTNFR1, and STREM-1 in the one or more test EVLP perfusate samples;
• c) optionally generating a transplant suitability score for the donor lung based on the one or more parameter values;
• d) comparing the one or more parameter values or optionally the transplant suitability score for the donor lung with a control or cut-off level;
• e) selecting the donor lung for transplant according to the one or more parameter values or the transplant suitability score.