Proximity-based labeling of biomolecular condensate microenvironments
OwnerDavid W.C. MacMillan, Chenmengxiao Pan +2inventors
InventorsDavid W.C. MacMillan, Chenmengxiao Pan, Steven Douglas Knutson, Sean Huth
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Abstract
Composites, systems and methods are described herein enabling the profiling of local microenvironments across various biomolecular condensates via proximity labeling. In some embodiments, a composite includes one or more biomolecular condensates comprising nucleic acids and/or functionally diverse proteins, and a transition metal catalyst coupled to the biomolecular condensate via interaction with at least one of the nucleic acids or functionally diverse proteins. Composites described herein, in some embodiments, are located in the intracellular environment.
Claims35
A composite comprising:
- one or more biomolecular condensates comprising nucleic acids and/or functionally diverse proteins;
- and
- a transition metal catalyst coupled to the biomolecular condensate via interaction with at least one of the nucleic acids or functionally diverse proteins.
A system for profiling interactome of a biomolecular condensate comprising:
- a protein labeling agent;
- and
- a composite, the composite including one or more biomolecular condensates comprising nucleic acids and/or functionally diverse proteins, and a transition metal catalyst coupled to the biomolecular condensate via interaction with at least one of the nucleic acids or functionally diverse proteins, wherein the transition metal catalyst has electronic structure permitting energy transfer to the protein labeling agent to provide a reactive intermediate.
A method of profiling microenvironments local to biomolecular condensates comprising:
- forming a composite comprising a transition metal catalyst coupled to a biomolecular condensate via interaction with a biomolecular species of the biomolecular condensate;
- activating a protein labeling agent to a reactive intermediate with the transition metal catalyst;
- and
- coupling the reactive intermediate to a protein or other biomolecules within a predetermined radius of the composite.
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Dependencies are inferred from the claim text.