System for adaptive spectral calibration

A system configured to provide spectral calibration for analyzing dye-labelled samples, the system comprising:

• one or more processors;
• and
• one or more hardware storage devices having stored thereon computer-executable instructions which are executable by the one or more processors to cause the system to at least:
• using a calibration sample having multiple known nucleic acids, determine sizes of two or more different nucleic acids;
• select a first nucleic acid with a first size, and determine a spectral profile associated with the first nucleic acid for a particular dye;
• using the spectral profile associated with the first nucleic acid, create a first dye matrix corresponding to the first nucleic acid;
• select a second nucleic acid with a second size, and determine a spectral profile associated with the second nucleic acid for the dye;
• using the spectral profile associated with the second nucleic acid, create a second dye matrix corresponding to the second nucleic acid;
• incorporate both the first dye matrix and the second dye matrix into a spectral calibration file;
• and
• write the spectral calibration file into an analytical instrument configured for analyzing dye-labelled samples.