Optically detected magnetic resonance with light-sheet microscopy

A system based on light sheet microscopy (LSM) and optically detected magnetic resonance (ODMR), comprising:

• a cylindrical lens CL;
• an objective lens O1 combined with the cylindrical lens CL and configured to generate a light sheet for exciting a sample from one side;
• a collection objective lens O2 configured to collect fluorescence generated by the sample;
• a galvo mirror configured to scan the light sheet in a first direction to realize three-dimensional (3D) imaging and sensing;
• an antenna configured to introduce microwave with frequency sweeping to the sample;
• and
• an image sensor configured to collect fluorescence of the sample under each microwave frequency point with a same exposure time to obtain ODMR signals;
• wherein the sample is fixed on a 3D stage and a position of the sample is adjustable, and
• wherein the cylindrical lens CL and the objective lens O1 are fixed on a stage along a second direction that is a propagation direction of a laser beam such that a position of the light sheet is adjustable along the second direction.