Two-strain system for producing oligosaccharides
AssigneeDsm IP Assets B.V.
InventorsManos PAPADAKIS, Ted JOHANSON, Getachew S. MOLLA, Niels BANKE
Life of this patent
Estimating the term…
Abstract
The present disclosure relates to a method for producing one or more oligosaccharides by co-culturing two different oligosaccharide producing strains. The method is useful for producing a balanced mixture of oligosaccharides, a selected oligosaccharide with reduced by-product formation and complex oligosaccharides, in particular complex fucosylated and sialylated HMOs. The co-cultivation is controlled by controlling the carbon source the individual strains can grow on.
Claims28
A method for producing one or more oligosaccharides having at least three monosaccharide units, comprising the steps of co-culturing a first and a second genetically modified microbial cell in a culture medium, wherein,
- a) the first genetically modified microbial cell produces, under fermentative conditions, a disaccharide or a first oligosaccharide of at least three monosaccharide units and wherein the genetically modified cell
- i) is capable of growing on a first carbon source while showing limited or no growth on a second carbon source, and
- ii) comprises one or more recombinant nucleic acid sequences encoding at least one glycosyltransferase;
- and
- iii) comprises at least one pathway to produce an activated sugar nucleotide from the first carbon source;
- and
- b) the second genetically modified microbial cell produces, under fermentative conditions, a second oligosaccharide of at least three monosaccharide units and wherein the genetically modified cell
- i) is capable of growing on the second carbon source while showing limited or no growth on the first carbon source, and
- ii) comprises one or more recombinant nucleic acid sequences encoding at least one glycosyltransferase, and
- iii) comprises a biosynthetic pathway to produce an activated sugar nucleotide from the second carbon source.
(canceled)
(canceled)
(canceled)
Dependencies are inferred from the claim text.