Biosynthesis of dihydrochalcones and dihydrostilbenoids

Abstract

Provided herein is a method of increasing production of a dihydrodiphenylpropanoid derivative compound (e.g., dihydrostilbenoid or dihydrochalcone compound) in a recombinant host cell. The method comprising: reducing or eliminating expression of a gene encoding a double-bond reductase polypeptide or activity of the double-bond reductase polypeptide encoded thereby; expressing a recombinant gene encoding a double-bond reductase selected from the group consisting of a first polypeptide, second polypeptide, third polypeptide, and combinations thereof. The first polypeptide comprises an amino acid sequence having at least 90% identity to the amino acid sequence as set forth in SEQ ID NO: 3, the second polypeptide comprises an amino acid sequence having at least 90% identity to the amino acid sequence as set forth in SEQ ID NO: 5, and the third polypeptide comprises an amino acid sequence having at least 90% identity to the amino acid sequence as set forth in SEQ ID NO: 7.

Dependencies are inferred from the claim text.