Exosomal loading using hydrophobically modified oligonucleotides
AssigneeUniversity of Massachusetts
InventorsAnastasia Khvorova, Neil Aronin, Marie Cecile Didiot, Reka Haraszti
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Abstract
In one aspect, the invention relates to a method of loading exosomes with oligonucleotide cargo, by incubating an oligonucleotide comprising one or more hydrophobic modifications with a population of exosomes for a period of time sufficient to allow loading of the exosomes with the oligonucleotide. Exosomes loaded with hydrophobically modified oligonucleotide cargo, and uses thereof, are also provided.
Claims18
A method of delivering an exogenous oligonucleotide to a subject, said method comprising administering to said subject a composition comprising a plurality of exosomes loaded with an oligonucleotide comprising one or more hydrophobic modifications, wherein the one or more hydrophobic modifications comprise a hydrophobic moiety conjugated to the oligonucleotide,
- wherein the hydrophobic moiety is a sterol, GM1, a lipid, a vitamin, or a peptide, or a combination thereof, and
- wherein the exosomes contain an average of about 1000-3000 oligonucleotides per exosome.
The method of claim 2, wherein at least 90% of the exosomes are loaded with the oligonucleotide.
Dependencies are inferred from the claim text.