Adeno-Associated Virus Production Platform
AssigneeMedImmune, LLC
InventorsAbdou NAGY, Albert SCHMELZER, Lina CHAKRABARTI, Soojin HAN
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Abstract
Provided herein is a novel rAAV-based HSV production method that includes two engineered cell lines: engineered CHO cells to produce multiple AAV serotypes and engineered BHK-21 to produce rHSV-1 stocks that are used in the production of rAAVs in CHO cells. The developed method provides a scalable, serum-free manufacturing platform.
Claims14
A baby hamster kidney cell that is adapted to grow in serum-free conditions, wherein the cell stably expresses a hamster codon-optimized herpes simplex virus-1 (HSV-1) ICP27 open reading frame comprising a deletion of non-essential infected-cell protein 27 (ICP27) elements.
A Chinese hamster ovary (CHO) cell that is adapted to grow in serum-free conditions, wherein the cell stably expresses one or more polypeptides necessary for entry and infection with herpes simplex virus-1 (HSV-1).
Dependencies are inferred from the claim text.