Aav1 vectors and uses thereof for treatment of otic indications

A method of expressing a polynucleotide that encodes Gap Junction Protein Beta 6 (Gib6) operably linked to a promoter in developed inner ear cells in a human subject, the method comprising administering to the developed inner ear cells of the human subject a serotype 1 adeno-associated virus (AAV1) vector harvested from a cell lysate, the AAV1 vector comprising the polynucleotide, wherein the administering is in an amount that transduces one or more inner ear cell types selected from the group consisting of vestibular dark cells, Border cells, inner phalangeal cells, inner pillar cells, outer pillar cells, first row Deiters' cells, second row Deiters' cells, third row Deiters' cells, Hensen's cells, Claudius cells, interdental cells, basal cells of the stria vascularis, intermediate cells of the stria vascularis, marginal cells of the stria vascularis, and fibrocytes.